The Acehan Lab

Research interests include development of single particle and tomography cryogenic electron microscopy methods for challenging samples. In particular, the research lab is interested in finding ways to improve three-dimensional models for low copy number biological structures within cells. Also, in collaboration with Dr. Sarah Bowman, the Director of the National Crystallization Center, we aim to increase success rates of micro-crystal electron diffraction experiments. At the Cryo Electron Microscopy Center, we provide high throughput screening and data collection for biological samples from many research groups.

Publications

• Riessland M., Kolisnyk B., Kim T.W., Cheng J., Ni J., Pearson J.A., Park E.J., Dam K., Acehan D., Ramos-Espiritu L.S., Wang W., Zhang J., Shim J., Ciceri G., Brichta L., Studer L., Greengard P. Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons (2019) Cell Stem Cell, 25, 514-530
• Govek, E., Wu, Z., Acehan D., Molina H., Rivera K., Zhu X., Fang Y., Tessier-Lavigne Marc, Hatten M.E. Cdc42 Regulates Neuronal Polarity during Cerebellar Axon Formation and Glial-Guided Migration (2018) iScience, 1, 35-48
• Wang W. J., Acehan D., Kao C. H., Jane W. N., Uryu K., & Tsou M. F. B. De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly. (2015) eLife, 4, e10586.
• Ronchi P., Tischer C., Acehan D., Pepperkok R. Positive feedback between Golgi membranes, microtubules and ER exit sites directs de novo biogenesis of the Golgi. (2014) J. Cell Sci, 127, 4620-4633.
• Acehan D., Santarella-Mellwig R., Devos D.P. A bacterial tubulovesicular network. (2014) J. Cell Sci, 127, 277-280
• Rehman A., Hemmert K.C, Ochi A., Jamal M., Henning J.R., Barilla R.,Quesada J.P., Zambirinis C.A., Tang K., Ego-Osuala M., Rao R.S., Greco S., Deutsch M., Narayan S., Pachter H.L., Graffeo C.S., Acehan D., Miller G. Role of Fatty-acid Synthesis in Dendritic Cell Generation and Function. (2013) J. Immunol., 190, 4640-4649
• Henning J.R., Graffeo C.S., Rehman A., Fallon N.C., Zambirinis C.P., OchiA., Barilla R., Jamal M., Deutsch M., Greco S., Ego-Osuala M., Bin Saeed U., Rao R.S., Badar S., Quesada J.P., Acehan D., Miller G. Dendritic cells limit fibro inflammatory injury in nonalcoholic steatohepatitis in mice (2013) Hepatology, 58, 589–602
• Schlame M., Acehan D., Berno B., Xu Y., Valvo S., Ren M., Stokes D.L., Epand R.M. The physical state of lipid substrates provides transacylation specificity for tafazzin (2012) Nature Chemical Biology, 8, 862-869
• Ibrahim J., Nguyen A.H., Rehman A., Ochi A., Jamal M., Graffeo C.S., Henning J.R., Zambirinis C.P., Fallon N.C., Barilla R., Badar S., Mitchell A., Rao R.S., Acehan D., Frey A.B., Miller G.  Dendritic Cell Populations with Different Concentrations of Lipid Regulate Tolerance and Immunity in Mouse and Human Liver (2012) Gastroentrology, 143(4), 1061-1072
• Phoon C.K. L., Acehan D., Schlame M., Stokes D.L., Edelman-Novemsky I., Yu D., Xu Y., Viswanathan N, Ren M.  Tafazzin Knockdown in Mice Leads to a Developmental Cardiomyopathy With Early Diastolic Dysfunction Preceding Myocardial Noncompaction (2012) J. of American Heart Association, 1, jah3-e000455
• Bedrosian A.S., Nguyen A.H., Hackman M., Connolly M.K., Malhotra A., Ibrahim J., Cieza-Rubio N.E., Henning J.R., Barilla R., Rehman A., Pachter H.L., Medina-Zea M.V., Cohen S.M., Frey A.B., Acehan D., Miller G. Dendritic Cells Promote Pancreatic Viability in Mice with Acute Pancreatitis (2011) Gastroenterology, 141, 1915-1926
• Acehan D., Malhotra A., Xu Y., Ren M., Stokes D.L. and Schlame M.  Cardiolipin Affects the Supramolecular Organization of ATP Synthase in Mitochondria (2011) Biophysical Journal, 100, 2184-2192
• Acehan D., Vaz F., Houtkooper R.H., James J., Moore V., Tokunaga C., Kulik W., Wansapura J., Toth M.J., Strauss A., and Khuchua Z.  Cardiac and skeletal muscle defects in a mouse model of human Barth syndrome (2011) J. Biol. Chem., 286, 899-908
• Xu F.Y., McBride H., Acehan D., Vaz F.M., Houtkooper R.H., Lee R.M, Mowat M.A. and Hatch G.M.  The dynamics of cardiolipin synthesis post-mitochondrial fusion. (2010) Biochimica et Biophysica Acta – Biomembranes, 1798,1577-1585
• Acehan D., Khuchua Z., Malhorta A., Houtkooper R.H., Kaufman J., Vaz F. M., Ren M., Rockman H.A., Stokes D.L., Schlame M. Distinct Effect of Tafazzin Deletion in Differentiated and Undifferentiated Mitochondria. (2009) Mitochondrion, 9, 86-95
• Akcora P., Liu H., Kumar S.K., Moll J., Li Y., Benicewicz B.C., Schadler L.S., Acehan D., Panagiotopoulos A.Z., Pryamitsyn V., Ganesan V., Ilavsky J., Thiyagarajan P., Colby R.H., and Douglas J.F.  Anisotropic self-assembly of spherical polymer-grafted nano particles. (2009) Nature Materials, 8, 354-359
• Acehan D., Petzold C., Gumper I., Sabatini D., Muller E., Cowin P., Stokes D.  Plakoglobin is required for effective Intermediate Filament Anchorage to Desmosomes. (2008) Journal of Investigative Dermatology, 128, 2665-2675
• Owen G., Acehan D., Derr K., Rice W., Stokes D. Comparative Electron Tomography of isolated Desmosomes. Biochemical Society (2008) Transactions, 36, 173-179
• Acehan D., Xu Y., Stokes D.L., Schlame M. Comparison of Lymphoblast Mitochondria from Normal Subjects and Patients with Barth Syndrome using Electron Microscopic Tomography. (2007) Laboratory Investigation, 87, 40-48
• Yu X., Wang L., Acehan D., Wang X., Akey C.W. Three-dimensional Structure of a Double Apoptosome formed by the Drosophila Apaf-1 Related Killer. (2006) Journal of Molecular Biology, 355, 577-589
• Yu X., Acehan D., Ménétret J-F, Booth C.R., Ludtke S.J., Riedl S.J., Shi Y., Wang X. and Akey C.W.
  A Structure of the Human Apoptosome at 12.8 Å Resolution Provides Insights into This Cell Death Platform. (2005) Structure, 13, 1725-1735
• Acehan D., Jiang X., Morgan D.G., Heuser J.E., Wang X. and Akey C.W.  Three-Dimensional Structure of the Apoptosome: Implications for Assembly, Procaspase-9 Binding and Activation. (2002) Molecular Cell, 9, 423-432