The Paz Lab
Aviv Paz Ph.D. is a protein biochemist that applies an array of complementary tools to study key-proteins that are involved in multiple diseases. To gain a deep and meaningful understanding of protein function, dynamics, and druggability, our lab first focuses on solving protein structures using X-ray crystallography or cryogenic electron microscopy. Using structures as templates, we then design experiments to test structure-derived hypotheses and further probe various functional aspects of the system at hand, such as ligand and drug binding specificities, kinetics, and protein dynamics. In order to do so, we employ methods from several experimental fields such as biochemistry, biophysics, molecular biology, physiology, and computational methods fostering multiple collaborations.
Representative Structures Solved by Dr. Paz.
Our Current Goals
Cells are enclosed by lipidic membranes that isolate them from each other and their environment. Membranes exert cellular sequestration due to their impermeability to most water-soluble molecules and ions. The ingress and egress of numerous molecules and ions to cells are governed by channels and transport proteins that are embedded in the membrane. As such, transporters and channels have tremendous importance in the normal physiology of all living organisms. Moreover, 60% of all drugs act on membrane proteins, while certain drugs are expelled from cells through specific transporters, rendering them ineffective. Our objectives are to gain a structural-based understanding of the dynamics of key-human transporters, relate these to functional aspects of the transporters, and harness these insights into the development of novel therapeutic approaches.
Transport Dynamics of Secondary Active Transporters
In order to bind and transport cargo between the two sides of the membrane, secondary active transporters are highly dynamic. They shift their conformations on fast time-scales in a thermodynamically reversible manner employing an alternating-access mechanism. In simplistic terms, a transporter shuttles its cargo from the extracellular milieu into the cell by sampling multiple conformations in which the ligand-binding site is situated at a cavity that is either exposed to the extracellular- or intracellular-space. When the cavity is open towards the extracellular space, driving ion- and ligand binding induce motions in the transporter that occlude the bound ligands, followed by an opening towards the intracellular space and finally, cargo release. After a successful transport event, the transporter isomerizes once again to an extracellular-open state and the transport cycle repeats over and over. Several factors as substrate and driving ion binding, and the membrane potential shift the equilibrium between the different conformers a transporter adopts.
We strive to characterize the most sampled conformations in the transport process, by generating structural snapshots of these, identify the principal pathways and the underlying kinetics by which transporters isomerize by utilizing spectroscopic, structural, and functional experimental systems. At this stage, our focus is on specific transporter targets related to the proliferation and treatment of cancer.
Ligand/Drug Binding Sites
Many transporters exhibit tissue-specific distributions. In some cancers, the expression levels of particular transporters are elevated. A striking example is the upregulation of sugar transporters from the GLUT family in several cancer types. GLUTs supply these tissues with abundant energy-sources that aid in the replication, metabolism, and ultimately the overall progression of the cancerous tissue. Structural studies of cancer-related transporters from different families in complex with known ligands could provide an atomic-resolution understanding of the specific interactions between the ligands and their portal into the cell, leading to a better definition of motifs that govern the binding and transport of the ligands. The general rule for transporters is that one transporter family binds and transports specific molecules. To do so, these transporters have a binding site that is highly specific to the transported ligand. But, there are a few exceptions to this rule. A few transporter families are multi-specific, i.e. they shuttle numerous ligands in or out of cells. One example is the efflux ABC-transporters that are responsible for drug resistance. In this case, the structural determinants of ligand selection deviate from the well-established binding site that is fine-tuned to bind one chemical family. We are interested in defining the structural motifs and architecture of both types of transporters – specific and multispecific of transporters that are involved in cancer progression and treatment.
Drug binding to transporters is another critical aspect that is extremely important for the treatment of multiple diseases and conditions. We are interested in the link between the binding mode of a drug to a transporter and its ensuing biological effects. Further, probing the differences of binding sites between isoforms or homologs could aid in drug design efforts to result in better drug specificities that lead to reduced side-effects. For these studies, we employ structural, functional, and biochemical/biophysical assays.
Regulation of OATP1B1 function by tyrosine kinase-mediated phosphorylation. Hayden E, Chen M, Pasquariello KZ, Gibson AA, Petti JJ, Shen S, Qu J, Ong SS, Chen T, Jin Y, Uddin ME, Huang KM, Paz A, Sparreboom A, Hu S, Sprowl JA. Clin Cancer Res, 2021 Mar 4, PMID:33664059.
- Substrate-bound outward-open structure of a Na+-coupled sialic acid symporter reveals a new Na+ site. Wahlgren WY, Dunevall E, North RA, Paz A, Scalise M, Bisignano P, Bengtsson-Palme J, Goyal P, Claesson E, Caing-Carlsson R, Andersson R, Beis K, Nilsson UJ, Farewell A, Pochini L, Indiveri C, Grabe M, Dobson RCJ, Abramson J, Ramaswamy S, Friemann R. Nat Commun. 2018, PMID:29717135.
- Conformational transitions of the sodium-dependent sugar transporter, vSGLT. Paz A, Claxton DP, Kumar JP, Kazmier K, Bisignano P, Sharma S, Nolte SA, Liwag TM, Nayak V, Wright EM, Grabe M, Mchaourab HS, Abramson J. Proc Natl Acad Sci USA. 2018, PMID: 29507231.
- Active site voltage clamp fluorometry of the sodium glucose cotransporter hSGLT1. Gorraitz E, Hirayama BA, Paz A, Wright EM, Loo DDF. Proc Natl Acad Sci USA. 2017, PMID: 29087341.
- A large Rab GTPase encoded by CRACR2A is a component of subsynaptic vesicles that transmit T cell activation signals. Srikanth S, Kim KD, Gao Y, Woo JS, Ghosh S, Calmettes G, Paz A, Abramson J, Jiang M, Gwack Y. Sci Signal. 2016, PMID: 27016526.
- Identification of a second substrate-binding site in solute-sodium symporters. Li Z, Lee AS, Bracher S, Jung H, Paz A, Kumar JP, Abramson J, Quick M, Shi L. J Biol Chem. 2015, PMID: 25398883.
- Structure-guided simulations illuminate the mechanism of ATP transport through VDAC1. Choudhary OP, Paz A, Adelman JL, Colletier JP, Abramson J, Grabe M. Nat Struct Mol Biol. 2014, PMID: 24908397.
- High resolution structure and double electron-electron resonance of the zebrafish voltage-dependent anion channel 2 reveal an oligomeric population. Schredelseker J, Paz A, López CJ, Altenbach C, Leung CS, Drexler MK, Chen JN, Hubbell WL, Abramson J. J Biol Chem. 2014, PMID: 24627492.
- The specific interaction of the photosensitizer methylene blue with acetylcholinesterase provides a model system for studying the molecular consequences of photodynamic therapy. Silman I, Roth E, Paz A, Triquigneaux MM, Ehrenshaft M, Xu Y, Shnyrov VL, Sussman JL, Deterding LJ, Ashani Y, Mason RP, Weiner L. Chem Biol Interact. 2013, PMID: 23159732.
- Structure of flagellar motor proteins in complex allows for insights into motor structure and switching. Vartanian AS, Paz A, Fortgang EA, Abramson J, Dahlquist FW. J Biol Chem. 2012, PMID: 22896702.
- Structural and functional characterization of the interaction of the photosensitizing probe methylene blue with Torpedo californica acetylcholinesterase. Paz A, Roth E, Ashani Y, Xu Y, Shnyrov VL, Sussman JL, Silman I, Weiner L. Protein Sci. 2012, PMID: 22674800.
- Flexibility of the flap in the active site of BACE1 as revealed by crystal structures and molecular dynamics simulations. Xu Y, Li MJ, Greenblatt H, Chen W, Paz A, Dym O, Peleg Y, Chen T, Shen X, He J, Jiang H, Silman I, Sussman JL. Acta Crystallogr D Biol Crystallogr. 2012, PMID: 22194329.
- Backbone and side chain NMR assignments for the intrinsically disordered cytoplasmic domain of human neuroligin-3. Wood K, Paz A, Dijkstra K, Scheek RM, Otten R, Silman I, Sussman JL, Mulder FA. Biomol NMR Assign. 2012, PMID: 21647611.
- The quaternary structure of amalgam, a Drosophila neuronal adhesion protein, explains its dual adhesion properties. Zeev-Ben-Mordehai T, Mylonas E, Paz A, Peleg Y, Toker L, Silman I, Svergun DI, Sussman JL. Biophys J. 2009, PMID: 19843464.
- Assessment of CASP8 structure predictions for template free targets. Ben-David M, Noivirt-Brik O, Paz A, Prilusky J, Sussman JL, Levy Y. Proteins. 2009, PMID: 19774550.
- The crystal structure of a complex of acetylcholinesterase with a bis-(-)-nor-meptazinol derivative reveals disruption of the catalytic triad. Paz A, Xie Q, Greenblatt HM, Fu W, Tang Y, Silman I, Qiu Z, Sussman JL. J Med Chem. 2009, PMID: 19326912.
- Amalgam, an axon guidance Drosophila adhesion protein belonging to the immunoglobulin superfamily: over-expression, purification and biophysical characterization. Zeev-Ben-Mordehai T, Paz A, Peleg Y, Toker L, Wolf SG, Rydberg EH, Sussman JL, Silman I. Protein Expr Purif. 2009, PMID: 18938249.
- Biophysical characterization of the unstructured cytoplasmic domain of the human neuronal adhesion protein neuroligin 3. Paz A, Zeev-Ben-Mordehai T, Lundqvist M, Sherman E, Mylonas E, Weiner L, Haran G, Svergun DI, Mulder FA, Sussman JL, Silman I. Biophys J. 2008 PMID: 18456828
- Operational definition of intrinsically unstructured protein sequences based on susceptibility to the 20S proteasome. Tsvetkov P, Asher G, Paz A, Reuven N, Sussman JL, Silman I, Shaul Y. Proteins. 2008, PMID: 17879262.
- Structural biology. It’s all in the symmetry. Abramson J, Paz A, Philipson KD. Science. 2012, PMID: 22323810.
- Structures of the prokaryotic galactose transporter vSGLT and their implications on alternating access mechanism in human SGLT1. Abramson J, Paz A, Vartanian AS. In Membrane Transport Mechanism 3D Structure and Beyond, 2014.
- Purification of Intrinsically Disordered Proteins. Paz A, Zeev-Ben-Mordehai T, Sussman J, Silman I. In Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation, 2010.