The Bowman Lab

My research interests include developing new methods for crystallization of biomolecules, new methods for detection of nanocrystals, and new methods for in situ X-ray data collection. My research lab is interested in developing new methods that combine crystallographic and spectroscopic approaches to answer fundamental questions about protein biochemistry, especially in proteins that contain metals. We are working to develop spectroscopic methods for single crystals (electron paramagnetic resonance, UV-visible microspectrophotometry, and energy dispersive X-ray spectroscopy for elemental analysis) to be used in parallel with crystallography.

At the Crystallization Center, we provide high-throughput screening for biomolecules to determine initial crystallization conditions, working with scientists around the world (www.getacrystal.org).

Software and scripting developed in the Bowman Lab are available on GitHub.

 

Our commitment to diversity

The Bowman Lab welcomes members regardless of race, religion, gender identification, sexual orientation, age, or disability status.

Bowman Lab News!

New perspective article about the role of structural biology in the fight against COVID-19!

In the midst of COVID-19, it has been awe-inspiring to see the response of the scientific community, including structural biologists. The IUCrJ perspective is a snapshot of the amazing work by the structural biology community in the past year using X-ray crystallography and cryoEM. Excited to collaborate with Eddie Snell and Miranda Lynch on this piece!

Congratulations to Max!

Max Dudek has been awarded a prestigious 2021 NSF Graduate Research Fellowship award to pursue graduate work!  He will be joining the Genomics and Computational Biology PhD prgram at UPenn!

Ethan’s paper is out!

Our new paper implementing MARCO automated crystal scoring is out in Journal of Applied Crystallography!  Great work, Ethan!

Code is open-source and available in GitHub!  Check out the links below:

Workshop and conference announcements!

Dr. Janet Newman and I are organizing a session on Getting the First Crystal at the virtual 2021 ACA meeting – check out the meeting program

Do you have a good ‘getting the first crystal’ story about a system you are working on? Do you have a strategy that works almost all of the time? We welcome submission of abstracts on these topics.

Dr. Jennifer Bridwell-Rabb and I are organizing a workshop for the 2021 SSRL/LCLS  Users’ Meeting on Metals in Structural Biology!  Details will be available soon.

Interviewed on Spectrum News!

See the story about our work in the Crystallization Center on SARS-CoV-2!

Hauptman-Woodward Research Institute Uses Crystallization To Search for COVID-19 Answers

Screening for SARS-CoV-2 protein crystals!

Screening for crystal growth of SARS-CoV-2 related samples at the Crystallization Center and getting great results!

Selected Publications

Lynch, ML, Snell, EH, Bowman, SEJ. (2021) Structural biology in the time of COVID-19: perspectives on methods and milestones. IUCrJ 8, 3, 335-341.

Holleman, ET, Duguid, E, Keefe, LJ, Bowman, SEJ. Polo: an open-source graphical user interface for crystallization screening. Journal of Applied Crystallography, 2021; 54(2):673-679.

Westerman, EL, Bowman, SEJ, Davidson, B, Davis, MC, Larson, ER, Sanford, C. Deploying Big Data to Crack the Genotype to Phenotype Code. Integrative and Comparative Biology, 2020; 60(2):385-396.

Lynch, ML, Dudek, MF, Bowman, SEJ.  A Searchable Database of Crystallization Cocktails in the PDB: Analyzing the Chemical Condition Space. Patterns 2020; 1(4), 100024.

Featured on journal cover

Bowman, SEJ, Backman, LRF, Bjork, RE, Andorfer, MC, Yori, S, Caruso, A, Stultz, CM, Drennan, CL. Solution structure and biochemical characterization of a spare part protein that restores activity to an oxygen-damaged glycyl radical enzyme.  Journal of Biological Inorganic Chemistry (2019). 24:817.

Nakashige, TG, Bowman, SEJ, Zygiel, EM, Drennan, CL, Nolan, EM. Biophysical Examination of the Calcium-Modulated Nickel-Binding Properties of Human Calprotectin Reveals Conformational Change in the EF-Hand Domains and His3Asp Site. Biochemistry, 2018; 57(28):4155-4164.

Bowman, SEJ*, Bridwell-Rabb, J*, Drennan, CL. Metalloprotein crystallography: More than a structure. Acc Chem Res. 2016 Apr 19;49(4):695-702.

*denotes co-first author publication.

ACS Editors’ Choice Article

Featured on journal cover

Gagnon, DM, Brophy, MB, Bowman, SEJ, Stich, TA, Drennan, CL, Britt, RD, Nolan, EM. Manganese binding properties of human calprotectin under conditions of high and low calcium: X-ray crystallographic and advanced electron paramagnetic resonance spectroscopic analysis. J Am Chem Soc. 2015 Mar 4;137(8):3004-16.

Galinato, MGI, Bowman, SEJ, Kleingardner, JG, Martin, S, Zhao, JY, Sturhahn, W, Alp, EE, Bren, KL, Lehnert, L. Effects of protein structure on iron-polypeptide vibrational dynamic coupling in cytochrome c.  Biochemistry. 2015 Feb 3;54(4):1064-76.

Kleingardner, JG, Bowman, SEJ, Bren, KL The influence of heme ruffling on porphyrin spin densities in cytochromes c probed by core heme carbon NMR. Inorg Chem. 2013 Nov 18;52(22):12933-46.

Galinato, MGI, Kleingardner, JG, Bowman, SEJ, Alp, EE, Zhao, J, Bren, KL, Lehnert, N. Heme-protein vibrational couplings in cytochromes c provide a dynamic link that connects the heme-iron and the protein surface. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8896-900.

Levin, BD, Can, M, Bowman, SEJ, Bren, KL, Elliott, SJ. Methionine ligand lability in bacterial monoheme cytochromes c: An electrochemical study. J Phys Chem B. 2011 Oct 13;115(40):11718-26.

Chung, JK, Thielges, MC, Bowman, SEJ, Bren, KL, Fayer, MD. Temperature dependent equilibrium native to unfolded protein dynamics and properties observed with IR absorption and 2D IR vibrational echo experimentsJ Am Chem Soc. 2011 May 4;133(17):6681-91.

Bowman, SEJ, Bren, KL. Variation and analysis of second sphere interactions and axial histidinate character in c-type cytochromes.  Inorg Chem. 2010 Sep 6;49(17):7890-7

Bowman, SEJ, Bren, KL. The chemistry and biochemistry of heme c: Functional bases for covalent attachment.  Nat Prod Rep. 2008 Dec;25(6):1118-30. doi: 10.1039/b717196j.

Kim, S, Chung, JK, Kwak, K, Bowman, SEJ, Bren, KL, Bagchi, B, Fayer, MD. Native and unfolded cytochrome c – Comparison of dynamics using 2D-IR vibrational echo spectroscopy.  J Phys Chem B. 2008 Aug 14;112(32):10054-63.

Michel, LV, Ye, T, Bowman, SEJ, Levin, BD, Hahn, MA, Russell, BS, Elliott, SJ, Bren, KL.  Heme attachment motif mobility tunes cytochrome c redox potentialBiochemistry. 2007 Oct 23;46(42):11753-60.

Education

Students in the Bowman Lab

Yana Shimanovich

Master’s Materials Design and Innovation UB 2020

Students in the Bowman Lab

Ethan Holleman Summer BioXFEL 2020

Current: Graduate school at UC Davis

Students in the Bowman Lab

Kailey Ferger Summer BioXFEL 2019
Current: Graduate school at UC Berkeley

Students in the Bowman Lab

Max Dudek HWI Summer 2018
Current: University of Pittsburgh; Soon: Graduate school at UPenn

Summer 2018 Interns

Sailing Trip

Summer 2019 Interns

Maid of the Mist

Summer 2019

Poster Session

Education

  • Cornell College, English Literature and Women’s Studies BSS
  • Metropolitan State College of Denver, Chemistry, BS
  • University of Rochester, Chemistry, MS, PhD
  • Massachusetts Institute of Technology, Postdoc
  • Los Alamos National Laboratory, Postdoc

Contact

Sarah Bowman, PhD

Associate Research Scientist
Hauptman-Woodward Medical Research Institute

Research Associate Professor
University at Buffalo Department of Biochemistry

T: 716.898.8623
sbowman@hwi.buffalo.edu

www.getacrystal.com