Human cells employ a large set of macromolecular machineries, also called biological nanomachines, to fulfill specific cellular functions. These nanomachines are made of individual building blocks. For example, proteins are made of amino acids. These building blocks change their spatial arrangements over time when the nanomachines use energy to fulfil certain actions. Our goal is to resolve such changes in spatial arrangements and suggest new ways to regulate these actions. Chemical or biological modulation of these actions will lead to new therapeutic strategies against human diseases caused by dysfunction of the target nanomachines.
The research programs in the Jiang laboratory are in three general directions —- molecular physiology of transmembrane proteins, structural basis underlying the functions of intracellular RNA-binding complexes and signaling, and nano-scale chemical engineering and its applications. First, we made two new discoveries in membrane physiology —- lipid-dependent gating of voltage-gated potassium (Kv) channels and identification of chromogranin B (CHGB) serving as the anion channel in the regulated secretory pathway. The lipid-dependent gating is closely tied to the life-threatening neurological diseases caused by lipid metabolic defects in human patients. The anion channel in regulated secretion had been missing for more than 40 years and is genetically linked to Type 2 diabetes, hypertension, neurodegenerative diseases and neuroendocrine cancers. The second direction focuses on RNA-binding complexes for RNA sensing, RNAi pathway, and the ON-OFF control of human telomerase holoenzyme. We are currently focusing on the kinetic regulation of human telomerase and its relation to cell aging and cancer development. The third direction is related to the new technologies I helped initiate: random spherically constrained (RSC) reconstruction, chemically functionalized nm-thick carbon (ChemiC) films, bead-supported unilamellar membranes (bSUMs) and carbon-supported planar unilamellar membranes (cPUMs). These technologies have extensive applications and are important for addressing our scientific questions.
A list of publications can be found in Pubmed
The Google Scholar page of the PI is at:
Mohammed Sayed, Ao Cheng, Andrew Ludlow, Jerry Shay, Woodring Wright and Qiu-Xing Jiang. Catalysis-dependent inactivation of human telomerase and its reactivation by intracellular telomerase-activating factors (iTAFs). J. Bio. Chem. 2019. pii: jbc.RA118.007234. doi: 10.1074/jbc.RA118.007234. [Epub ahead of print]. PMID:31186347
Qiu-Xing Jiang. Cholesterol-dependent gating effects on ion channels. Adv Exp Med Biol. 2019; 1115:167-190. doi: 10.1007/978-3-030-04278-3_8. PMID: 30649760.
Qiu-Xing Jiang. Structural variability in the RLR-MAVS pathway and sensitive detection of viral RNAs. Medicinal Chemistry. 2019. doi: 10.2174/1573406415666181219101613. PMID 30569868
Gaya Yadavp, Hui Zhengp, Qing Yangp, Lauren Douma, Linda Bloom, and Qiu-Xing Jiang. Secretory granule protein chromogranin B forms an anion channel in membrane. Life Science Alliance. Life Science Alliance. 24 September 2018. DOI: 10.26508/lsa.201800139. PMID 30456382
Mulik RS, Zheng H, Pichumani K, Ratnakar J, Jiang, Q-X., Corbin IR. Elucidating the structural organization of a novel low-density lipoprotein nanoparticle reconstituted with docosahexaenoic acid. (2017) Chemistry and physics of lipids. 204:65-75. doi:10.1016/j.chemphyslip.2017.03.007. PMID 28342772.
Hui Zheng*, Sungsoo Lee*, Marc C. Llaguno, and Qiu-Xing Jiang. bSUM: a bead-supported unilamellar membrane system enabling unidirectional insertion of membrane proteins into giant vesicles. (2016) Journal of General Physiology. 147(1):77-93; (* equal first authors). PMID: 26712851
Cai X, Chen J, Xu H, Liu S, Jiang QX, Halfmann R, Chen ZJ. (2014) Prion-like polymerization underlies signal transduction in antiviral immune defense and inflammasome activation. Cell. 156(6) : 1207-22. PMID: 24630723.
Hui Xu, Xiaojing He, Hui Zheng, Zhiheng Yu, Brian Borkowski, Xuewu Zhang*, Zhijian J. Chen* and Qiu-Xing Jiang*. Structural basis for the prion-like MAVS filaments in antiviral innate immunity. (2014) eLife. 3:e01489. (* co-corresponding authors).
Marc Llaguno, Hui Xu, Liang Shi, Nian Huang, Hong Zhang, Qinghua Liu, and Qiu-Xing Jiang. Chemically functionalized nanometer-thick carbon films for single molecule imaging. (2014) J. Struct. Biology. Jan 21. pii: S1047-8477(14)00007-0. doi: 10.1016/j.jsb.2014.01.006.
Sohini Mukherjee, Hui Zheng, Mehabaw Derebe, Keith Carrie L. Partch, Darcy Rollins, Josep Rizo, Qiu-Xing Jiang*, and Lora V. Hooper*. Antibacterial membrane attach by a pore-forming C-type lectin. (2014) Nature, 505(7481):103-7. Online version available on Nov. 20, 2013. doi: 10.1038/nature12729. (*co-senior authors).
Alpay B. Seven, Kyle D. Brewer, Liang Shi, Qiu-Xing Jiang, and Josep Rizo. Prevalent mechanism of membrane bridging by synaptotagmin-1. (2013). PNAS 110(34):E3243-52.
Anju Sreelatha,Terry L. Bennett, Hui Zheng, Qiu-Xing Jiang, Kim Orth, and Vincent J. Starai. Vibrio parahaemolyticus Effector VopQ forms a gated, outward rectifying channel that disrupts host ion homeostasis. (2013) PNAS 110, 11559-11564.
Sungsoo Lee, Hui Zheng, Liang Shi, and Qiu-Xing Jiang. Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies (2013). J. Vis. Exp. 77, e50436.
Pilong Li, Sudeep Banjade, Hui-Chun Cheng, Soyeon Kim, Stone Chen, Liong Guo, Marc Llaguno, Javoris V. Hollingsworth, David S. King, Salman F. Banani, Paul S. Russo, Qiu-Xing Jiang, B. Tracy Nixon, and Michael K. Rosen. Phase Transitions in the Assembly of Multi-valent signaling proteins. (2012). Nature, 483:336-340.
Fajian Hou, Lijun Sun, Hui Zheng, Brian Skaug, Qiu-Xing Jiang, and Zhijian J. Chen. MAVS forms functional prion-like aggregates to activate and propagate antiviral innate immune response. (2012). Cell, 146:448-461 (cover story).
Yi Xu, Alpay Seven, Lijing Su, Qiu-Xing Jiang, and Josep Rizo. Membrane bridging and hemifusion by denaturate Munc18. (2011). PLoS One, 6:e22012.
Hui Zheng, Weiran Liu, Lingyan Anderson, and Qiu-Xing Jiang. Lipid-dependent gating of a voltage-gated potassium channel. (2011). Nature Communications 2:250 doi: 10.1038/ncomms1254.
Derk Ninns, SungKyung Lee, Christopher L. Hilton, Qiu-Xing Jiang, Joel M. Goodman. Seipin is a discrete homooligomer. (2010). Biochemistry 49(50): 10747-10755.
Daniel Schmidt*, Qiu-Xing Jiang*, and Roderick MacKinnon. Phospholipids and the origin of cationic gating charges in voltage sensors. Nature 444:775-779, 2006. (* equal contribution).
Qiu-Xing Jiang, Da-Neng Wang, and Roderick MacKinnon. Electron microscopic analysis of KvAP voltage-dependent K+ channels in an open conformation. Nature 430:806-10, 2004.
Qiu-Xing Jiang, Edwin C. Thrower, David W. Chester, Barbara E. Ehrlich, and Fred J. Sigworth. Three-dimensional structure of Type 1 inositol 1,4,5-trisphosphate receptor at 24 Å resolution. EMBO J., 21(14):3575-3581, 2002.
Qiu-Xing Jiang, David W. Chester, and Fred J. Sigworth. Spherical reconstruction: a method for structure determination of membrane proteins from cryo-EM images. J. Struct. Biol., 133:119-131, 2001.
Qiu-Xing Jiang. Spherical reconstruction: a novel method for structure determination from cryo-EM images of membrane proteins in small vesicles. Ph.D. dissertation, Yale University, 2001.
Qiu-Xing Jiang, Kunsheng Hu and Wei Wang. pH-dependence of the interaction between melittin and bacteriorhodopsin. Science in China (Series C), 9:362-372, 1996.
Qiu-Xing Jiang, Kunsheng Hu and Wei Wang. The effect of melittin on the kinetic spectra of bacteriorhodopsin photocycle intermediate O (in Chinese). Chinese Science Bulletin, 41:647-650, 1996.
Qiu-Xing Jiang and Kungsheng Hu. The biphasic decay of bacteriorhodopsin photocycle intermediate M: the effect of melittin and its point mutants (in Chinese). Chinese Science Bulletin, 40:940-943, 1995.
Qiu-Xing Jiang and Kungsheng Hu. The pH dependence of the interaction between melittin and bacteriorhodopsin (in Chinese). Science in China (Series B), 25:1071-1078, 1995.
Qiu-Xing Jiang, Kun-Sheng Hu, and Hua Shi. Interactions of both melittin and its site-specific mutants with bacteriorhodopsin of Halobacterium halobium: sites of electrostatic interaction on melittin. Photochemistry Photobiology, 60:175-178, 1994.
Qiu-Xing Jiang and Tamir Gonen. The influence of lipids on the voltage-gated ion channels. Curr. Opin. Struct. Biol. 22(4):529-536, 2012. PMID: 22483432 / PMCID: PMC3408884
Qiu-Xing Jiang and Zhijian J. Chen. Structural insights into the activation of RIG-I, a nanosensor for viral RNAs. EMBO Rep. 13(1):7-8, 2011 (invited mini-review).
Qiu-Xing Jiang and Kunsheng Hu. Recent progress in the study of bacteriorhodopsin (review in Chinese). Progress in Biochemistry and Biophysics, 23:397-402, 1996.
Qiu-Xing Jiang. The discovery of split genes won the 1993 Nobel Prize at Physiology and Medicine (in Chinese). Progress in Biochemistry and Biophysics, 21:182-183, 1994.
Qiu-Xing Jiang (2020) Introduction: new tools for challenges in membrane biology. Chapter 1, pages 1-8. In: “New techniques for studying biomembranes” ed. By Qiu-Xing Jiang. CRC Press, Taylor & Francis Group, LLC. Boca Raton, London & New York.
Gaya Yadav and Qiu-Xing Jiang (2020) Reconstituted membrane systems for assaying membrane proteins in controlled lipid environments. Chapter 6, pages 93-122. In: “New techniques for studying biomembranes” ed. By Qiu-Xing Jiang. CRC Press, Taylor & Francis Group, LLC. Boca Raton, London & New York.
Qiu-Xing Jiang (2018) Lipid-dependent gating of ion channels. Book chapter in “Protein-lipid interactions: perspectives, techniques, and challenges” edited by Catala Angel, Argentina. Nova Science Publishers, Inc. https://www.easons.com/protein-lipid-interactions-angel-catala-9781536131253.
Qiu-Xing Jiang and Liang Shi: CryoEM study of ion channels in “Handbook of Ion Channels” edited by M.C. Trudeau and J. Zheng, CRC press, 2013.
Qiu-Xing Jiang (ed.) (2020) New techniques for studying biomembranes. Series title: “Method in signal transduction series” by Joseph Eichberg Jr., Michael X. Zhu, and Harpreet Singh”. CRC Press, Taylor & Francis Group, Boca Raton, London, New York.