Development and synthesis of photocaged GPCR ligands for time-resolved structural biology

The Hauptman-Woodward Institute seeks a postdoctoral research scientist in the field of synthetic organic chemistry. The successful candidate will work within the National Science Foundation’s BioXFEL Science and Technology center (www.bioxfel.org), a consortium of 13 research labs across the USA dedicated to the study of protein dynamics by time-resolved structural biology. Specifically, the work will comprise the design, synthesis and characterization of novel ligands of G-protein coupled receptors bearing photocleavable groups to be used for rapid and efficient protein function activation in crystals and solutions. The project is highly synergistic with other areas of research beyond synthetic chemistry, including biochemistry, protein crystallography, cryo-EM and pump-probe spectroscopy. Time-resolved structural biology has provided the first “stop-motion movies” of proteins at work at atomic resolution. The ability to observe the structural intermediates explored by molecular machines yields unprecedented insight into their dynamics and plasticity. This information is invaluable for the understanding of both global and local structure-activity relationships and can be used to guide the design of novel drugs or the engineering of proteins with optimized properties. With current high-brilliant X-ray sources available, and extensive advances in sample production, delivery and data collection techniques, the main hurdle is the protein activation step. Photocaging strategies fill this gap and are in high demand by the structural biology community.

We are looking for a highly motivated synthetic chemist with an interest in Chemical Biology to join our interdisciplinary team. The position will be based at the Hauptman-Woodward Medical Research Institute in Buffalo, NY (www.hwi.buffalo.edu) and is a collaboration with the Liu, Fromme and Ros groups at the Biodesign Institute, Arizona State University. The candidate must hold a doctorate or equivalent in organic chemistry or related discipline by the time of appointment. Previous experience in designing and modifying synthetic routes is highly desirable. Prior knowledge about protein structure, medicinal chemistry or biochemistry would be highly advantageous.

Benefits:The Institute’s benefits package includes very generous health insurance, life insurance and parking. In addition we offer affordable vision and dental plans as well as Flexible spending and dependent care accounts. The Institute provides a retirement plan which consists of a 6% employer contribution after one year of service.

 Initial application deadline: May 14th 2020 To apply, please send a cover letter and a CV containing a list of publications list to Dr. Diana Monteiro (dmonteiro@hwi.buffalo.edu).

About HWI and Buffalo, NY:

HWI has celebrated more than 60 years as an independent research institute and is continually growing. Located in Buffalo, New York, HWI is at the heart of the growing Buffalo Niagara Medical Campus and partners with the University at Buffalo, Roswell Park Comprehensive Cancer Center, University of Rochester and numerous others. It also has links to industry and operates the IMCA-CAT beamline at the Advanced Photon Source outside of Chicago.
Buffalo offers a vast array of community organizations which add to the richness of the area and also offer people many opportunities to grow and contribute. Housing is one of the most affordable in the United States.$

Postdoctoral Research Scientist

Protein Production and Purification 

The Hauptman-Woodward Institute seeks a lab technician to join the Institute’s efforts in combating COVID-19. The successful candidate will be joining a collaborative project between Dr. Diana Monteiro (Hauptman-Woodward) and Dr. Thomas Grant (University at Buffalo) employing biochemical and structural biology techniques to elucidate protein-ligand interactions of COVID19 relevant proteins. 

We are looking for a motivated applicant keen to help progress this timely and very relevant project. The technician will be performing standard biochemical and molecular biology tasks within a protein production and purification pipeline as well as support general lab operation. The work does not involve contact any infectious samples.

The position will be based at the Hauptman-Woodward Medical Research Institute in Buffalo, NY. Extensive safety measures are in place at the Institute to ensure the safe working of staff. The candidate must hold a college degree in biochemistry or related discipline by the time of appointment. Prior knowledge of basic biochemical and molecular biology techniques is required and basic laboratory experience would be highly advantageous.

Benefits: As this position is part-time, free parking is provided but health insurance is not offered.

Preferred start date: June 1st 2020

Initial application deadline: May 14th 2020 To apply, please send a CV detailing any previous experience and a short paragraph about your interest in the position to Dr. Diana Monteiro (dmonteiro@hwi.buffalo.edu). The position is part-time – 18.75 hours per week.