Proprietary Crystallization Screening

The Crystallization Center has worked with industrial users since the Center began operations more than 20 years ago. Samples are screened against 1,536 different crystallization conditions. For the soluble screen, the assay includes an incomplete factorial based PEG/Salt/Buffer screen (688 cocktails) and chemically complementary screens purchased from Hampton Research which includes: Crystal Screen HT, Index, PEGRx HT, SaltRx HT, PEG/Ion HT, Slice pH, Ionic liquid, Polymer Screen, Silver-bullets, and others (848 cocktails). The membrane screen, samples regions of detergent phase space particularly relevant to crystallization (1344 cocktails) with the Hampton Research PEGRx screen (96 cocktails), and the Molecular Dimensions MemGold screen (96 cocktails).  Typically the experimental error for delivery of cocktails and/or protein is < 5% (most often the error is < 1%) and we use control experiments to rapidly identify and address any issues that arise.

Sample requirement: Currently, we require 500 μL of protein sample in a labeled 1.5 ml microcentrifuge tube. We recommend that you send only high purity, non-aggregated, stable samples including all chemicals required for stability. To ensure that the crystallization conditions dominate use low (<25mM) buffer concentration. Avoid phosphate buffer (which forms insoluble salts). A recommended concentration is 10 mg/ml where solubility permits but both lower and significantly higher concentrations have proved successful. We do not recommend lyophilizing your protein.

Proprietary research studies are kept confidential. Information will not be publicly disclosed without the express written consent of the client.

Our philosophy is to provide capabilities at the highest standards for quality, reliability, efficiency, and productivity while providing confidentiality and expert support for challenging experiments. We want you to be successful and help that by providing cost-effective access to world-class research capabilities and maintaining competitiveness. We understand industry needs and can provide a platform of services offering solutions.