Reproducing Crystallization Screening Center Results

Reproducing HWI crystallization screening hits

We recommend a Greiner 72-well microbatch plate, treated hydrophilic (Hampton Research HR3‐121).

Set up 5 replications each of experiments (1) through (3) by following the protocol described below.

  1. 1μL protein + 2μL cocktail
  2. 1μL protein + 1μL cocktail
  3. 2μL protein + 1μL cocktail

Step 1. Add the cocktail drop to each of the five replicate experiment wells in a row of the plate. You can use a microscope to watch the drop deliveries to each set up if desired.

Step 2. Add the protein drop (do not mix) by touching the pipette tip to the cocktail drop