A book entitled Biological Small Angle Scattering: Theory and Practice, by Eaton Lattman, Thomas Grant, and Edward Snell, is part of an International Union of Crystallography series that provides in-depth knowledge of specialized topics. The technique of small-angle solution scattering has been revolutionized in the last two decades. Exponential increases in computing power, parallel algorithm development, and the development of synchrotron, free-electron X-ray sources, and neutron sources have combined to allow new classes of studies for biological specimens. These include time-resolved experiments in which functional motions of proteins are monitored on a picosecond timescale and the first steps towards determining actual electron density fluctuations within particles. In addition, more traditional experiments involving the determination of size and shape, and contrast matching that isolate substructures such as nucleic acid, have become much more straightforward to carry out, and simultaneously require much less material. These new capabilities have sparked an upsurge of interest in solution scattering on the part of investigators in related disciplines. Thus, this book seeks to guide structural biologists to understand the basics of small-angle solution scattering in both the X-ray and neutron case, to appreciate its strengths, and to be cognizant of its limitations. It is also directed at those who have a general interest in its potential. The book focuses on three areas: theory, practical aspects and applications, and the potential of developing areas. It is an introduction and guide to the field but not a comprehensive treatment of all the potential applications.
Biological Small Angle X-ray scattering – Theory and Practice
