Artem Evdokimov, PhD

I have worked for Procter & Gamble Pharmaceuticals and Pfizer, where I used structural biology as a tool to assist in the development of potential new treatments for infectious diseases, CNS ailments, cardiovascular conditions and cancer. Most recently I led Structure and Design team at Monsanto, where my work enabled the development of novel traits to protect plants against insect predation, to elicit herbicide tolerance, or to enhance yield. Today, I am CEO of HarkerBIO, a spin-off of HWI.
Artem received a dual PhD in Chemistry and Structural Biology from the Weizmann Institute of Science in Israel. He then spent two years at the National Institutes of Health (NCI-Frederick in the lab of Dr. David Waugh) where he studied Yersinia pestis, the bacterial pathogen that causes the notorious Bubonic Plague.
Evdokimov AG, Moshiri F, Sturman EJ, Rydel TJ, Zheng M, Seale JW, Franklin S. Structure of the full-length insecticidal protein Cry1Ac reveals intriguing details of toxin packaging into in vivo formed crystals. Protein Sci. 2014 Nov;23(11):1491-7. doi: 10.1002/pro.2536. Epub 2014 Sep 2. PubMed PMID: 25139047; PubMed Central PMCID: PMC4241100.

John M. Humphrey, Eddie Yang, Christopher W. am Ende, Eric P. Arnold, Jenna L. Head, Stephen Jenkinson, Lorraine A. Lebel, Spiros Liras, Jayvardhan Pandit, Brian Samas, Felix Vajdos, Samuel P. Simons, Artem Evdokimov, Mahmoud Mansour and Frank S. Menniti. “Small-molecule phosphodiesterase probes: discovery of potent and selective CNS-penetrable quinazoline inhibitors of PDE1” Med. Chem. Commun, 5, 1290-1296, 2014.

Evdokimov AG, Mekel M, Hutchings K, Narasimhan L, Holler T, McGrath T, Beattie B, Fauman E, Yan C, Heaslet H, Walter R, Finzel B, Ohren J, McConnell P, Braden T, Sun F, Spessard C, Banotai C, Al-Kassim L, Ma W, Wengender P, Kole D, Garceau N, Toogood P, Liu J. Rational protein engineering in action: the first crystal structure of a phenylalanine tRNA synthetase from Staphylococcus haemolyticus. J Struct Biol. 2008 Apr;162(1):152-69. Epub 2007 Nov 13. PubMed PMID: 18086534.

Holler TP, Evdokimov AG, Narasimhan L. Structural biology approaches to antibacterial drug discovery. Expert Opin Drug Discov. 2007 Aug;2(8):1085-101. doi: 10.1517/17460441.2.8.1085. PubMed PMID: 23484874.

Evdokimov AG, Pokross M, Walter R, Mekel M, Cox B, Li C, Bechard R, Genbauffe F, Andrews R, Diven C, Howard B, Rastogi V, Gray J, Maier M, Peters KG. Engineering the catalytic domain of human protein tyrosine phosphatase beta for structure-based drug discovery. Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1435-45. Epub 2006 Nov 23. PubMed PMID: 17139078.

Evdokimov AG, Pokross M, Walter RL, Mekel M, Barnett BL, Amburgey J, Seibel WL, Soper SJ, Djung JF, Fairweather N, Diven C, Rastogi V, Grinius L, Klanke C, Siehnel R, Twinem T, Andrews R, Curnow A. Serendipitous discovery of novel bacterial methionine aminopeptidase inhibitors. Proteins. 2007 Feb 15;66(3):538-46. PubMed PMID: 17120228.

Warshakoon NC, Wu S, Boyer A, Kawamoto R, Sheville J, Renock S, Xu K, Pokross M, Evdokimov AG, Walter R, Mekel M. A novel series of imidazo[1,2-a]pyridine derivatives as HIF-1alpha prolyl hydroxylase inhibitors. Bioorg Med Chem Lett. 2006 Nov 1;16(21):5598-601. Epub 2006 Sep 8. PubMed PMID: 16962772.

Evdokimov AG, Pokross ME, Egorov NS, Zaraisky AG, Yampolsky IV, Merzlyak EM, Shkoporov AN, Sander I, Lukyanov KA, Chudakov DM. Structural basis for the fast maturation of Arthropoda green fluorescent protein. EMBO Rep. 2006 Oct;7(10):1006-12. Epub 2006 Aug 25. PubMed PMID: 16936637; PubMed Central PMCID: PMC1618374.

Warshakoon NC, Wu S, Boyer A, Kawamoto R, Sheville J, Renock S, Xu K, Pokross M, Zhou S, Winter C, Walter R, Mekel M, Evdokimov AG. Structure-based design, synthesis, and SAR evaluation of a new series of 8-hydroxyquinolines as HIF-1alpha prolyl hydroxylase inhibitors. Bioorg Med Chem Lett. 2006 Nov 1;16(21):5517-22. Epub 2006 Aug 22. PubMed PMID: 16931007.

Warshakoon NC, Wu S, Boyer A, Kawamoto R, Sheville J, Bhatt RT, Renock S, Xu K, Pokross M, Zhou S, Walter R, Mekel M, Evdokimov AG, East S. Design and synthesis of substituted pyridine derivatives as HIF-1alpha prolyl hydroxylase inhibitors. Bioorg Med Chem Lett. 2006 Nov 1;16(21):5616-20. PubMed PMID: 16908149.

Warshakoon NC, Wu S, Boyer A, Kawamoto R, Renock S, Xu K, Pokross M, Evdokimov AG, Zhou S, Winter C, Walter R, Mekel M. Design and synthesis of a series of novel pyrazolopyridines as HIF-1alpha prolyl hydroxylase inhibitors. Bioorg Med Chem Lett. 2006 Nov 1;16(21):5687-90. PubMed PMID: 16908145.

Amarasinghe KK, Evdokimov AG, Xu K, Clark CM, Maier MB, Srivastava A, Colson AO, Gerwe GS, Stake GE, Howard BW, Pokross ME, Gray JL, Peters KG. Design and synthesis of potent, non-peptidic inhibitors of HPTPbeta. Bioorg Med Chem Lett. 2006 Aug 15;16(16):4252-6. Epub 2006 Jun 12. Erratum in: Bioorg Med Chem Lett. 2008 Aug 15;18(16):4745.. Evidokimov, Artem G [corrected to Evdokimov, Artem G]. PubMed PMID: 16759857.

Klopfenstein SR, Evdokimov AG, Colson AO, Fairweather NT, Neuman JJ, Maier MB, Gray JL, Gerwe GS, Stake GE, Howard BW, Farmer JA, Pokross ME, Downs TR, Kasibhatla B, Peters KG. 1,2,3,4-Tetrahydroisoquinolinyl sulfamic acids as phosphatase PTP1B inhibitors. Bioorg Med Chem Lett. 2006 Mar 15;16(6):1574-8. Epub 2006 Jan 4. PubMed PMID: 16386905.

Derewenda U, Mateja A, Devedjiev Y, Routzahn KM, Evdokimov AG, Derewenda ZS, Waugh DS. The structure of Yersinia pestis V-antigen, an essential virulence factor and mediator of immunity against plague. Structure. 2004 Feb;12(2):301-6. PubMed PMID: 14962390.

Zhdanov AS, Phan J, Evdokimov AG, Tropea JE, Kapust RB, Li M, Wlodawer A, Waugh DS. [Tobacco etch virus proteinase: crystal structure of the active enzyme and its inactive mutant]. Bioorg Khim. 2003 Sep-Oct;29(5):457-60. Russian. PubMed PMID: 14601399.

Evdokimov AG, Phan J, Tropea JE, Routzahn KM, Peters HK, Pokross M, Waugh DS. Similar modes of polypeptide recognition by export chaperones in flagellar biosynthesis and type III secretion. Nat Struct Biol. 2003 Oct;10(10):789-93. Epub 2003 Sep 7. PubMed PMID: 12958592.

Phan J, Zdanov A, Evdokimov AG, Tropea JE, Peters HK 3rd, Kapust RB, Li M, Wlodawer A, Waugh DS. Structural basis for the substrate specificity of tobacco etch virus protease. J Biol Chem. 2002 Dec 27;277(52):50564-72. Epub 2002 Oct 10. PubMed PMID: 12377789.

Evdokimov AG, Tropea JE, Routzahn KM, Waugh DS. Three-dimensional structure of the type III secretion chaperone SycE from Yersinia pestis. Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):398-406. Epub 2002 Feb 21. PubMed PMID: 11856824.

Bucher MH, Evdokimov AG, Waugh DS. Differential effects of short affinity tags on the crystallization of Pyrococcus furiosus maltodextrin-binding protein. Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):392-7. Epub 2002 Feb 21. PubMed PMID: 11856823.

Evdokimov AG, Tropea JE, Routzahn KM, Waugh DS. Crystal structure of the Yersinia pestis GTPase activator YopE. Protein Sci. 2002 Feb;11(2):401-8. PubMed PMID: 11790850; PubMed Central PMCID: PMC2373448.

Evdokimov AG, Anderson DE, Routzahn KM, Waugh DS. Unusual molecular architecture of the Yersinia pestis cytotoxin YopM: a leucine-rich repeat protein with the shortest repeating unit. J Mol Biol. 2001 Sep 28;312(4):807-21. PubMed PMID: 11575934.

Evdokimov AG, Tropea JE, Routzahn KM, Copeland TD, Waugh DS. Structure of the N-terminal domain of Yersinia pestis YopH at 2.0 A resolution. Acta Crystallogr D Biol Crystallogr. 2001 Jun;57(Pt 6):793-9. Epub 2001 May 25. PubMed PMID: 11375498.

Evdokimov AG, Anderson DE, Routzahn KM, Waugh DS. Structural basis for oligosaccharide recognition by Pyrococcus furiosus maltodextrin-binding protein. J Mol Biol. 2001 Jan 26;305(4):891-904. PubMed PMID: 11162100.

Evdokimov AG, Anderson DE, Routzahn KM, Waugh DS. Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of YopM, an essential virulence factor extruded by the plague bacterium Yersinia pestis. Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1676-9. PubMed PMID: 11092944.

Tsoumpra MK, Muniz JR, Barnett BL, Kwaasi AA, Pilka ES, Kavanagh KL, Evdokimov A, Walter RL, Von Delft F, Ebetino FH, Oppermann U, Russell RG, Dunford JE. The inhibition of human farnesyl pyrophosphate synthase by nitrogen-containing bisphosphonates. Elucidating the role of active site threonine 201 and tyrosine 204 residues using enzyme mutants. Bone. 2015 Dec;81:478-86. doi: 10.1016/j.bone.2015.08.020. Epub 2015 Aug 28. PubMed PMID: 26318908; PubMed Central PMCID: PMC4652608.

Duff SM, Rydel TJ, McClerren AL, Zhang W, Li JY, Sturman EJ, Halls C, Chen S, Zeng J, Peng J, Kretzler CN, Evdokimov A. The enzymology of alanine aminotransferase (AlaAT) isoforms from Hordeum vulgare and other organisms, and the HvAlaAT crystal structure. Arch Biochem Biophys. 2012 Dec 1;528(1):90-101. doi: 10.1016/j.abb.2012.06.006. Epub 2012 Jun 29. PubMed PMID: 22750542.

Schumacher MA, Min J, Link TM, Guan Z, Xu W, Ahn YH, Soderblom EJ, Kurie JM, Evdokimov A, Moseley MA, Lewis K, Brennan RG. Role of unusual P loop ejection and autophosphorylation in HipA-mediated persistence and multidrug tolerance. Cell Rep. 2012 Sep 27;2(3):518-25. doi: 10.1016/j.celrep.2012.08.013. Epub 2012 Sep PubMed PMID: 22999936; PubMed Central PMCID: PMC4831868.

Dounay AB, Anderson M, Bechle BM, Campbell BM, Claffey MM, Evdokimov A, Evrard E, Fonseca KR, Gan X, Ghosh S, Hayward MM, Horner W, Kim JY, McAllister LA, Pandit J, Paradis V, Parikh VD, Reese MR, Rong S, Salafia MA, Schuyten K, Strick CA, Tuttle JB, Valentine J, Wang H, Zawadzke LE, Verhoest PR. Discovery of Brain-Penetrant, Irreversible Kynurenine Aminotransferase II Inhibitors for Schizophrenia. ACS Med Chem Lett. 2012 Jan 25;3(3):187-92. doi: 10.1021/ml200204m. eCollection 2012 Mar 8. PubMed PMID: 24900455; PubMed Central PMCID: PMC4025856.

Han S, Zaniewski RP, Marr ES, Lacey BM, Tomaras AP, Evdokimov A, Miller JR, Shanmugasundaram V. Structural basis for effectiveness of siderophore-conjugated monocarbams against clinically relevant strains of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2010 Dec 21;107(51):22002-7. doi: 10.1073/pnas.1013092107. Epub 2010 Dec 6. PubMed PMID: 21135211; PubMed Central PMCID: PMC3009787.

Evdokimov A, Voznesensky I, Fennell K, Anderson M, Smith JF, Fisher DA. New kinase regulation mechanism found in HipBA: a bacterial persistence switch. Acta Crystallogr D Biol Crystallogr. 2009 Aug;65(Pt 8):875-9. doi: 10.1107/S0907444909018800. Epub 2009 Jul 17. PubMed PMID: 19622872.

Mochalkin I, Miller JR, Evdokimov A, Lightle S, Yan C, Stover CK, Waldrop GL. Structural evidence for substrate-induced synergism and half-sites reactivity in biotin carboxylase. Protein Sci. 2008 Oct;17(10):1706-18. doi: 10.1110/ps.035584.108. Epub 2008 Aug 25. PubMed PMID: 18725455; PubMed Central PMCID: PMC2548373.

Deprèle S, Kashemirov BA, Hogan JM, Ebetino FH, Barnett BL, Evdokimov A, McKenna CE. Farnesyl pyrophosphate synthase enantiospecificity with a chiral risedronate analog, [6,7-dihydro-5H-cyclopenta[c]pyridin-7-yl(hydroxy)methylene]bis(phosphonic acid) (NE-10501): Synthetic, structural, and modeling studies. Bioorg Med Chem Lett. 2008 May 1;18(9):2878-82. doi: 10.1016/j.bmcl.2008.03.088. Epub 2008 Apr 8. PubMed PMID: 18434151.

Sabat M, VanRens JC, Laufersweiler MJ, Brugel TA, Maier J, Golebiowski A, De B, Easwaran V, Hsieh LC, Walter RL, Mekel MJ, Evdokimov A, Janusz MJ. The development of 2-benzimidazole substituted pyrimidine based inhibitors of lymphocyte specific kinase (Lck). Bioorg Med Chem Lett. 2006 Dec 1;16(23):5973-7. Epub 2006 Sep 25. PubMed PMID: 16997556.

Hopkins CR, O'neil SV, Laufersweiler MC, Wang Y, Pokross M, Mekel M, Evdokimov A, Walter R, Kontoyianni M, Petrey ME, Sabatakos G, Roesgen JT, Richardson E, Demuth TP Jr. Design and synthesis of novel N-sulfonyl-2-indole carboxamides as potent PPAR-gamma binding agents with potential application to the treatment of osteoporosis. Bioorg Med Chem Lett. 2006 Nov 1;16(21):5659-63. Epub 2006 Aug 21. PubMed PMID: 16919947.

Greis KD, Zhou S, Burt TM, Carr AN, Dolan E, Easwaran V, Evdokimov A, Kawamoto R, Roesgen J, Davis GF. MALDI-TOF MS as a label-free approach to rapid inhibitor screening. J Am Soc Mass Spectrom. 2006 Jun;17(6):815-22. Epub 2006 Apr 17. PubMed PMID: 16616859.

Peters KG, Davis MG, Howard BW, Pokross M, Rastogi V, Diven C, Greis KD, Eby-Wilkens E, Maier M, Evdokimov A, Soper S, Genbauffe F. Mechanism of insulin sensitization by BMOV (bis maltolato oxo vanadium); unliganded vanadium (VO4) as the active component. J Inorg Biochem. 2003 Aug 1;96(2-3):321-30. PubMed PMID: 12888267.

Evdokimov A, Gilboa AJ, Koetzle TF, Klooster WT, Schultz AJ, Mason SA, Albinati A, Frolow F. Structures of furanosides: geometrical analysis of low-temperature X-ray and neutron crystal structures of five crystalline methyl pentofuranosides. Acta Crystallogr B. 2001 Apr;57(Pt 2):213-20. PubMed PMID: 11262436.



Patents

JC Lamb and AG Evdokimov, “Compositions and methods for custom site-specific DNA recombinases”, US20140193915, 2014

James A. Baum, Allen T. Christian, Artem Evdokimov, Farhad Moshiri, Lisa Marie Weaver, Haitao Zhang “Compositions and methods for the production and delivery of double stranded RNA”, US 20140271559, 2014.

Richard Masaru Kawamoto, Shengde Wu, Artem G. Evdokimov, Kenneth D. Greis, Angelique Sun Boyer, Namal C. Warshakoon “Prolyl hydroxylase inhibitors and methods of use”, US 20140057892, 2014.

David J. Bowen, Catherine Chay, Artem Evdokimov, Megan N. Schroder, Rachael N. Slightom, Uma Sukuru, Nengbing Tao, Andrew M. Wollacott “Insect Inhibitory Toxin Family Active Against Hemipteran and/or Lepidopteran Insects”, US 20130097735, 2013

Klopfenstein S.R., Maier M.B., Jones D.R., GrayJ.L., Pokross M.E., Peters K.G., Evdokimov A. G. “Tetrahydroisoquinolnyl sulfamic acids”. U.S. Pat. Appl. Publ. 2005, US20050154011.

Evdokimov A.G., Pokross M.E., “Three dimensional coordinates of HPTPbeta ”,U.S. Pat. Appl. Publ. 2004, US20040077065.

Artem Evdokimov, PhD
T: 716 898 8688
artem.evdokimov@harkerbio.com