High-Throughput Crystallization Screening Center

Our Expertise – Your Results

The High-Throughput Crystallization Screening Center performs crystal-growth screening experiments in 1,536-well microassay plates for both soluble and membrane biological macromolecules. You send the sample, we do the rest.

Upcoming Screening Run Dates and Deadlines

July and August 2018 (updated 07/03/2018)

July Membrane Screen (Note: We will not be scheduling a Membrane Protein Screening Run for August)

      • Reservation Deadline: Friday, July 6th
      • Package Acceptance: Tues July 10th – Thurs July 12th
      • Plate Setup: Tues July 10th – Thurs July 12th

July Standard Screen

      • Reservation Deadline: Friday, July 14th
      • Package Acceptance: Tues July 17th – Thurs July 26th
      • Plate Setup: Tues July 17th – Thurs July 19th and Tues July 24th – Thurs July 26th

August Standard Screen

      • Reservation Deadline: Friday, Aug10th
      • Package Acceptance: Tues Aug 14th – Thurs Aug 23rd
      • Plate Setup: Tues Aug 14th – Thurs Aug 16th and Tues Aug 21st – Thurs Aug 23rd

      Click ‘Get a Crystal‘ for details. Staff and Support

In the Literature!

For each crystallization experiment run at the HTCSC, 13,824 images are generated. Currently, a user has to visually inspect each of those images to determine if a crystal has formed or not, a major investment of person-time.

The Machine Recognition of Crystallization Outcomes (MARCO) initiative, a multi-institutional collaboration, has assembled a massive database of annotated images of macromolecular crystallization experiments (including those from the HTCSC). The MARCO initiative has developed an innovative algorithm to apply machine learning to these images, taking a major step forward in automatic image analysis of crystallization experiments. The paper (Classification of crystallization outcomes using deep convolutional neural networks) is just out in PLoS ONE!

We are working to implement this algorithm for HTCSC users for automatic classification of images – stay tuned!

From February 2000, the laboratory has been used to set up over 24 million crystallization experiments on >16,000 biological macromolecules. Over 1,000 laboratories worldwide have used and benefited from the service.

  • At the HTCSC 1,536 different chemical conditions are sampled representing many of the commercially available crystallization screens. Each condition is imaged visually over a period of six weeks. Second Order Non-linear Imaging of Chiral Crystals (SONICC) and UV Two Photon Emitted Fluorescence (UV-TPEF) imaging of crystallization screening also take place to identify biological crystals not picked up visually or those obscured by precipitate.
  • Color images, and SONICC and UV-TPEF images are integrated with the analysis software provided as part of the service. Lead crystallization conditions can be identified and the complete set of results used to analyze the samples solubility phase diagram.
  • Current list of crystallization cocktails.

HTCSC Policies and User Fees

This service is for academic and other not-for-profit users only. Our commercial users go through ‘the structure people‘, our friends at HarkerBIO (www.harkerbio.com).

New at HWI HTCSC!

New submission form!

Starting in May 2018 we will have a new online submission form.

      • The new online forms are now available!
      • To make a reservation, please email htslab@hwi.buffalo.edu by the reservation deadline (see above for current dates) to reserve a space in the queue.  Include the following information in your reservation email:
        • User name and contact information
        • PI/Institution name and contact information
        • Number of samples you will be sending, as well as approximate date you will be shipping the sample(s)

        You will receive an email confirmation from us for your reservation.  Once your sample is ready to ship, please fill out the online submission form including payment information.

Subscription program and price changes

      • Beginning May 2018 there will be a nominal increase for an individual 1,536 crystallization screen to $400 (from $375) to account for increased costs
      • We are offering a trial subscription service for May – October 2018!
        • 5 crystallization screening experiments for $1900
        • Trial subscription for 5 screen is pre-paid for the 6-month period between May – October 2018
        • Any additional crystallization screening experiments (past 5) will cost subscribers $370 per screen
      • Please contact sbowman@hwi.buffalo.edu for details, with questions or to set up a subscription